Journal: World Journal of Diabetes
Article Title: Mechanism of the epidermal growth factor receptor in promoting endothelial cell dysfunction in gestational diabetes mellitus
doi: 10.4239/wjd.v16.i6.105173
Figure Lengend Snippet: EIF4A3 binding to long noncoding RNAs H19 to increase the expression of epidermal growth factor receptor promotes endothelial cell dysfunction in mice with gestational diabetes mellitus. A: Western blot showing epidermal growth factor receptor expression in mouse placental tissue; B: Pathological changes in mouse placental tissue observed with hematoxylin-eosin staining; C: Immunohistochemistry of cluster of differentiation 31 in mouse placental tissue; D: Cell apoptosis in mouse placental tissue detected by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-nick end labelling staining; E: Western blot showing the expression of endothelial cell dysfunction markers in mouse placental tissue. a P < 0.05. b P < 0.01. c P < 0.001. EGFR: Epidermal growth factor receptor; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; OE- H19 : Overexpression H19 ; sh- EIF4A3 : Short hairpin RNA targeting EIF4A3 ; GDM: Gestational diabetes mellitus; TUNEL: Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-nick end labelling; DAPI: 4’,6-diamidino-2-phenylindole; CD31: Cluster of differentiation 31; VCAM-1: Vascular cell adhesion molecule-1; TNF-α: Tumor necrosis factor-α; VEGF-A: Vascular endothelial growth factor-A; ICAM-1: Intercellular cell adhesion molecule-1.
Article Snippet: Then, 50 μL of terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-nick end labelling (TUNEL) detection solution (consisting of 2 μL of terminal deoxynucleotidyl transferase enzyme + 48 μL of fluorescein-labeled 2’-deoxyuridine 5’-triphosphate solution) (C1089, Beyotime, China) was carefully added to the sample in a dropwise manner, and the sample was coated with slides and incubated in the dark in a humidified chamber at 37 °C for 1 hour.
Techniques: Binding Assay, Expressing, Western Blot, Staining, Immunohistochemistry, Over Expression, shRNA, TUNEL Assay
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